The PROPAGASI IN VITRO KALIANDRA MERAH (Calliandra calothyrsus Meisn.) I: PERKECAMBAHAN BIJI DAN INISIASI TUNAS DARI EKSPLAN HIPOKOTIL DAN NODUS
Keywords:
Biofuel, Calliandra, Micropropagation, Seed germination, Shoot multiplication
Abstract
The red calliandra is a fuel wood crops that gave benefit for people. Recently, it is getting wide attention due to its important use as biofuel in industrial scale. The massive propagation is needed to meet industrial needs. Beside the use of conventional seedling production technique, the use of tissue culture for mass propagation is one of the alternatives to address this problem. This research was conducted to develop standard protocol for micropropagation of red calliandra through tissue culture. Here, the first step for that purpose was reported. The MS medium was used for basal media treatment. The seed source was stored for eight months under 5oC. This research was carried out in two stages i.e. 1) seed germination test and 2) shoot initiation and multiplication treatment. Each stage was arranged in experimental method. The result showed that seed were pre-treated with warm water (40oC) gave 62.5% germination under red light when compared to control (37.5%, pre-treated with water at 25oC under white light) three days after planting. Conversely, there is no striking differences of germinated seed percentages between light treatment at 25oC. Furthermore, it was found that addition of 2 mg/L BAP gave better result in shoot initiation and number of shoots formed from nodal explants after four weeks in treatment media. These results indicated that mass propagation of red calliandra can be done through tissue culture where multiplication stage is one of the important steps to produce multiple shoots only from single nodal explant.
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References
Chamberlain JR. 2000. Improving seed production in Calliandra calothyrsus, A field manual for researchers and extension workers. United Kingdom (UK): Oxford Forest Institute Oxford.
E Silveira FAO, Fernandes F, Fernandes GW. 2005. Light and temperature influence on seed germination of Calliandra fasciculata Benth. (Leguminosae). Lundiana 6: 95-97.
Gantait S, Mukherjee E. 2021. Tissue culture‑based genetic improvement of fava bean (Vicia faba L.): Analysis on previous achievements and future perspectives. Applied Microbiology and Biotechnology 105: 6531-6546.
George EF, De Klerk GJ. 2008. The component of tissue culture media I: Macro- and micro- nutrients. In: George EF, Hall MA, De Klerk GJ, editors. Plant Propagation by Tissue Culture 3rd Edition Volume 1. The Background. The Netherlands (NL): Springer Dordrecht. p. 65-114.
Goldbio [Internet]. 2019. Benzylaminopurine (stock colution TD-S Revision 2.0). Missouri, US: Gold Biotechnology; [updated 2019 Oct 17; cited 2023 Nov 01] Available from: https://goldbio.com/documents/1380/Benzylaminopurine%20Stock%20Solution.pdf
Greenway, MB, Philips IC, Lloyd MN, Hubstenberger JF, Philips GC. 2012. A nutrient medium for diverse applications and tissue growth of plant species in vitro. In Vitro Cellular & Developmental Biology – Plant 48: 403-410.
Hiti-Bandaralage JCA, Hayward A, Mitter N. 2017. Micropropagation of avocado (Persea americana Mill.). American Journal of Plant Sciences 8: 2898-2921.
Hussain A, Qarshi IA, Nazir H, Ullah I. 2012. Plant tissue culture: Current status and opportunities. In: Leva A, Rinaldi L, editors. Recent Advances in Plant in vitro Culture. United Kingdom (UK): IntechOpen. p. 1-28. Available at doi: doi: 10.5772/50568.
International Rice Research Institute [Internet]. 2013. Grain storage and pest management.Los Banos PH: Postharvest Unit, CESD, International Rice Research Institute (IRRI); [updated 2013 Oct; cited 2023 Nov 01] Available from: http://www.knowledgebank.irri.org/images/docs/training-manual-grain-storage.pdf
Idris M, Soe N, Jiang L, Kiyota S, Hidema J, Iino M. 2021. UV-B signalling in rice: Response identification, gene expression profiling and mutant isolation. Plant, Cell & Environment 44: 1468-1485
Ismail S, Purwanto RH. 2014. Potensi biomassa dan karbon jenis kaliandra merah (Calliandra calothyrsus) dan peluangnya dalam pengurangan emisi gas karbon dioksida. Indonesia (ID): Universitas Gadjah Mada Yogyakarta.
Ismail AY, Supartono T, Kusmana C, Sumekar Y, Aminudin S, Hendrayana Y, Nurlaila A. 2022. Phenology of flowering and fruiting of calliandra (Calliandra spp.) species in submontane forest, Indonesia. Research on Crops 23: 172-179.
Jayasuriya KMGG, Phartyal SS. 2023. Dormancy, germination, and associated seed ecological traits of 25 Fabaceae species from northern India. Plant Biology (early view): 13589. Available with doi: https://doi.org/10.1111/plb.13589.
Joseph N, Siril EA, Nair GM. 2011. An efficient in vitro propagation methodology for Annatto (Bixa orellana L.). Physiology and Molecular Biology of Plants 17: 263-270.
Katta YM, Kamara MM, Abd El-Aty MS, Elgamal WH, Soleiman RM, Mousa KM, Ueno T. 2019. Effect of storage temperature on storage efficacy, germination, and physical characters of some paddy rice cultivars during different storage periods. Journal of the Faculty of Agriculture, Kyushu University 64: 61-69.
Kieber JJ, Schaller GE. 2014. Cytokinins. The Arabidopsis Book 12: e0168. Available with doi: https://doi.org/10.1199/tab.0168.
Lui K, Baskin JM. Baskin CC, Bu H, Liu M, Liu W, Du G. 2011. Effect of storage conditions on germination of seeds of 489 species from high elevation grasslands of the Eastern Tibet Plateau and some implications for climate change. American Journal of Botany 98: 12-19.
Lumakto G [Internet]. 2018. Kaliandra merah, tanaman pembangkit tenaga listrik: kompasiana.com; [cited 28 May 2023]. Available from: https://www.kompasiana.com/girilu/5b3dc8985e1373602321bab2/kaliandra-merah-tanaman-pembangkit-tenaga-listrik
Mitrofanova I, Lesnikova-Sedoshenko N, Mitrofanova O, Smykov A, Chelombit S. 2020. Comparative studies of in vitro regeneration capacity in some breeding forms of Prunus persica (L.) Batsch. In: BIO Web of Conferences 24. Proceedings: 2020. International Conferences “Plant Diversity: Status, Trends, Conservation Concept”. p. 00055. Available with doi: https://doi.org/10.1051/bioconf/20202400055.
Neumann KH, Kumar A, Imani J. 2009. Plant cell and tissue culture – a tool in biotechnology. Germany (DE): Springer-Verlag Berlin Heidelberg.
Park S. 2021. Plant tissue culture technique and experiments 4th Edition. United Kingdom (UK): Academic Press (Elsevier) Oxford.
Putri VA, Sugiyono, Prayoga L, Prasetyo R, Hilary S. 2022. The application of two steps culture in agarwood, Aquilaria malaccensis, in vitro culture improves microshoots induction and development. Scripta Biologica 9: 1-5.
Shaheen A, Ali M, Ahmad N, Dewir YH, El-Hendawy S, Abd-El Gawad AM. 2020. Micropropagation of licorice (Glycyrrhiza glabra L.) by using intermediate nodal explants. Chilean Journal of Agricultural Research 80: 326-333.
Shekhawat MS, Kannan N, Manokari M, Ravindran CP. 2015. In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures. 2015. Journal of Genetic Engineering and Biotechnology 13: 209-214.
Stewart J, Mulawarman, Roshetko JM, Powell MH. 2001. Produksi dan pemanfaatan kaliandra (Calliandra calothyrsus): Pedoman lapang. Indonesia (ID): International Centre for Research in Agroforestry (ICRAF) Bogor.
Suliasih. 1985. Sekilas uraian mengenai kaliandra. Buletin Kebun Raya 6: 133-136.
Tun W, Yoon J, Jeon JS, An G. 2021. Infuence of climate change on flowering time. Journal of Plant Biology 64: 193-203.
Tyub S, Dar SA, Lone IM, Mir AH. 2021. A robust in-vitro protocol for shoot multiplication of Echinacea angustifolia. Current Plant Biology 28: 100221. Available with doi: https://doi.org/10.1016/j.cpb.2021.100221.
Wawrzyniak MK, Michalak M, Chmielarz P. 2020. Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants. Annals of Forest Science 77: 58. Available with doi: https://doi.org/10.1007/s13595-020-00963-z.
Xing Y, Wen D, Qin C, Jiang W, Wu Z, Qin X. 2020. Effect of low temperature storage on seed germination and seedling growth of dragon fruit. Agricultural Biotechnology 9: 73-78.
E Silveira FAO, Fernandes F, Fernandes GW. 2005. Light and temperature influence on seed germination of Calliandra fasciculata Benth. (Leguminosae). Lundiana 6: 95-97.
Gantait S, Mukherjee E. 2021. Tissue culture‑based genetic improvement of fava bean (Vicia faba L.): Analysis on previous achievements and future perspectives. Applied Microbiology and Biotechnology 105: 6531-6546.
George EF, De Klerk GJ. 2008. The component of tissue culture media I: Macro- and micro- nutrients. In: George EF, Hall MA, De Klerk GJ, editors. Plant Propagation by Tissue Culture 3rd Edition Volume 1. The Background. The Netherlands (NL): Springer Dordrecht. p. 65-114.
Goldbio [Internet]. 2019. Benzylaminopurine (stock colution TD-S Revision 2.0). Missouri, US: Gold Biotechnology; [updated 2019 Oct 17; cited 2023 Nov 01] Available from: https://goldbio.com/documents/1380/Benzylaminopurine%20Stock%20Solution.pdf
Greenway, MB, Philips IC, Lloyd MN, Hubstenberger JF, Philips GC. 2012. A nutrient medium for diverse applications and tissue growth of plant species in vitro. In Vitro Cellular & Developmental Biology – Plant 48: 403-410.
Hiti-Bandaralage JCA, Hayward A, Mitter N. 2017. Micropropagation of avocado (Persea americana Mill.). American Journal of Plant Sciences 8: 2898-2921.
Hussain A, Qarshi IA, Nazir H, Ullah I. 2012. Plant tissue culture: Current status and opportunities. In: Leva A, Rinaldi L, editors. Recent Advances in Plant in vitro Culture. United Kingdom (UK): IntechOpen. p. 1-28. Available at doi: doi: 10.5772/50568.
International Rice Research Institute [Internet]. 2013. Grain storage and pest management.Los Banos PH: Postharvest Unit, CESD, International Rice Research Institute (IRRI); [updated 2013 Oct; cited 2023 Nov 01] Available from: http://www.knowledgebank.irri.org/images/docs/training-manual-grain-storage.pdf
Idris M, Soe N, Jiang L, Kiyota S, Hidema J, Iino M. 2021. UV-B signalling in rice: Response identification, gene expression profiling and mutant isolation. Plant, Cell & Environment 44: 1468-1485
Ismail S, Purwanto RH. 2014. Potensi biomassa dan karbon jenis kaliandra merah (Calliandra calothyrsus) dan peluangnya dalam pengurangan emisi gas karbon dioksida. Indonesia (ID): Universitas Gadjah Mada Yogyakarta.
Ismail AY, Supartono T, Kusmana C, Sumekar Y, Aminudin S, Hendrayana Y, Nurlaila A. 2022. Phenology of flowering and fruiting of calliandra (Calliandra spp.) species in submontane forest, Indonesia. Research on Crops 23: 172-179.
Jayasuriya KMGG, Phartyal SS. 2023. Dormancy, germination, and associated seed ecological traits of 25 Fabaceae species from northern India. Plant Biology (early view): 13589. Available with doi: https://doi.org/10.1111/plb.13589.
Joseph N, Siril EA, Nair GM. 2011. An efficient in vitro propagation methodology for Annatto (Bixa orellana L.). Physiology and Molecular Biology of Plants 17: 263-270.
Katta YM, Kamara MM, Abd El-Aty MS, Elgamal WH, Soleiman RM, Mousa KM, Ueno T. 2019. Effect of storage temperature on storage efficacy, germination, and physical characters of some paddy rice cultivars during different storage periods. Journal of the Faculty of Agriculture, Kyushu University 64: 61-69.
Kieber JJ, Schaller GE. 2014. Cytokinins. The Arabidopsis Book 12: e0168. Available with doi: https://doi.org/10.1199/tab.0168.
Lui K, Baskin JM. Baskin CC, Bu H, Liu M, Liu W, Du G. 2011. Effect of storage conditions on germination of seeds of 489 species from high elevation grasslands of the Eastern Tibet Plateau and some implications for climate change. American Journal of Botany 98: 12-19.
Lumakto G [Internet]. 2018. Kaliandra merah, tanaman pembangkit tenaga listrik: kompasiana.com; [cited 28 May 2023]. Available from: https://www.kompasiana.com/girilu/5b3dc8985e1373602321bab2/kaliandra-merah-tanaman-pembangkit-tenaga-listrik
Mitrofanova I, Lesnikova-Sedoshenko N, Mitrofanova O, Smykov A, Chelombit S. 2020. Comparative studies of in vitro regeneration capacity in some breeding forms of Prunus persica (L.) Batsch. In: BIO Web of Conferences 24. Proceedings: 2020. International Conferences “Plant Diversity: Status, Trends, Conservation Concept”. p. 00055. Available with doi: https://doi.org/10.1051/bioconf/20202400055.
Neumann KH, Kumar A, Imani J. 2009. Plant cell and tissue culture – a tool in biotechnology. Germany (DE): Springer-Verlag Berlin Heidelberg.
Park S. 2021. Plant tissue culture technique and experiments 4th Edition. United Kingdom (UK): Academic Press (Elsevier) Oxford.
Putri VA, Sugiyono, Prayoga L, Prasetyo R, Hilary S. 2022. The application of two steps culture in agarwood, Aquilaria malaccensis, in vitro culture improves microshoots induction and development. Scripta Biologica 9: 1-5.
Shaheen A, Ali M, Ahmad N, Dewir YH, El-Hendawy S, Abd-El Gawad AM. 2020. Micropropagation of licorice (Glycyrrhiza glabra L.) by using intermediate nodal explants. Chilean Journal of Agricultural Research 80: 326-333.
Shekhawat MS, Kannan N, Manokari M, Ravindran CP. 2015. In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures. 2015. Journal of Genetic Engineering and Biotechnology 13: 209-214.
Stewart J, Mulawarman, Roshetko JM, Powell MH. 2001. Produksi dan pemanfaatan kaliandra (Calliandra calothyrsus): Pedoman lapang. Indonesia (ID): International Centre for Research in Agroforestry (ICRAF) Bogor.
Suliasih. 1985. Sekilas uraian mengenai kaliandra. Buletin Kebun Raya 6: 133-136.
Tun W, Yoon J, Jeon JS, An G. 2021. Infuence of climate change on flowering time. Journal of Plant Biology 64: 193-203.
Tyub S, Dar SA, Lone IM, Mir AH. 2021. A robust in-vitro protocol for shoot multiplication of Echinacea angustifolia. Current Plant Biology 28: 100221. Available with doi: https://doi.org/10.1016/j.cpb.2021.100221.
Wawrzyniak MK, Michalak M, Chmielarz P. 2020. Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants. Annals of Forest Science 77: 58. Available with doi: https://doi.org/10.1007/s13595-020-00963-z.
Xing Y, Wen D, Qin C, Jiang W, Wu Z, Qin X. 2020. Effect of low temperature storage on seed germination and seedling growth of dragon fruit. Agricultural Biotechnology 9: 73-78.
Published
2024-04-26
How to Cite
Idris, M., Asman, A., Sorel, D., Joniarti, E., Mohtar, U., Harmailis, H., Nefri, J., & Salivia, S. (2024). The PROPAGASI IN VITRO KALIANDRA MERAH (Calliandra calothyrsus Meisn.) I: PERKECAMBAHAN BIJI DAN INISIASI TUNAS DARI EKSPLAN HIPOKOTIL DAN NODUS. Bio-Lectura : Jurnal Pendidikan Biologi, 11(1), 32-45. https://doi.org/10.31849/bl.v11i1.16877
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