https://journal.unilak.ac.id/index.php/jip/issue/feedJurnal Ilmiah Pertanian2024-03-14T14:31:57+00:00Indra Purnama[email protected]Open Journal Systems<p>Jurnal Ilmiah Pertanian (JIP) is an open-access journal that publishes original research articles on agricultural advances and various other agricultural resources at the local level to increase global awareness.. <br><br>Initially, the Jurnal Ilmiah Pertanian (JIP) was published twice yearly, every February and August. However, starting with Volume 19, 2022, Jurnal Ilmiah Pertanian (JIP) is a triannual journal published every March, July, and Dec. It is published electronically via the journal website. ISSN (Print) 1829-8346 ISSN (Online) 2502-5988</p>https://journal.unilak.ac.id/index.php/jip/article/view/18856Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia2024-03-14T14:31:57+00:00Adyatma Irawan Santosa[email protected]Adenisa Hanifah Irbati[email protected]Geby Nandita Cahyaning Pratiwi[email protected]Antama Surwadinata[email protected]Putri Laeshita[email protected]Krisnanda Surya Dharma[email protected]Reko Saputra Jaya[email protected]Annisa Lisa Andriyani[email protected]Chilya Qurrota A'yun[email protected]Chusnu Naura Syifa Fatika[email protected]<p>Chili pepper, tomato, and squash cultivated in Magelang Regency, Central Java Province, Indonesia showed severe viral symptoms. Three samples were taken then molecularly tested against begomovirus, potyvirus, tobamovirus, and polerovirus. However, PCR test confirmed only begomovirus infections. BLAST analysis concluded that the chili pepper and tomato isolates were pepper yellow leaf curl Indonesia virus (PepYLCIV) while the squash isolate was squash leaf curl China virus (SLCCNV). The 552 bp partial AV1 gene sequences of the three isolates were given NCBI GenBank acc nos. OR924278-80. PepYLCIV OR924279 and OR924280 formed a subgroup with LC542629 from Bali in the phylogenetic tree constructed using MEGA11, and shared 95.8 – 96.7% identities at nucleotide (nt) and 98.4 – 99.5% at amino acid (aa) levels according to Sequence Demarcation Tool v1.2 software. Meanwhile, SLCCNV OR924278 was clustered, and shared 95.8 – 96.7% nt and 98.4 – 99.5% aa identities with three Malaysian isolates (MW248685, MW248687, and MW248689). Different plant species inoculated with SLCCNV OR924278 remained symptomless up to four weeks observation, suggesting that the isolate is not mechanically transmissible. This study contributed additional knowledge on molecular variation while expanding hosts and distribution of PepYLCIV and SLCCNV in Central Java Province.</p>2024-03-14T00:00:00+00:00Copyright (c) 2024 Jurnal Ilmiah Pertanian