PENGARUH KOSENTRASI HORMON KINETIN DAN IAA PADA KULTUR JARINGAN TANAMAN MANGROVE (Rhizophora apiculate BL)
Abstract
Mangroves (Rhizophora apiculata) are one of the plants that grow in mangrove forests. The significant degradation of mangrove forests in Indonesia requires effective and efficient rehabilitation efforts. Efforts to provide superior seeds in a short time and in large quantities, one of which is through tissue culture. ZPT (Growth Regulator) Kinetin and IAA in balance can stimulate the morphogenesis process in the explant. This study used a 4×4 factorial randomized complete (RAL) design with 3 replications. The first factor is the concentration of kinetin. 0 mg/L, 1 mg/L, 3 mg/L, and 5 mg/L. The second factor is IAA concentrations of 0 mg/L, 0.5 mg/L, 1 mg/L, and 1.5 mg/L. The parameters observed were explant growth time, explant growth percentage, and bud height. The results showed an interaction with the plant growth time factor, and the best plant growth rate was achieved using the kinetin treatment of 5 mg/L and the IAA treatment of 1.5 mg/L, while the best shoot height factor was found in the kinetin treatment of 5 mg/L. l and IAA 1 mg/L treatment concentrate.
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References
Dewi, I.R. 2008. Peranan dan fungsi fitohormon bagi pertumbuhan tanaman. Fakultas Pertanian, Universitas Padjajaran.
Emilda, E. 2020. Potensi bahan-bahan hayati sebagai sumber zat pengatur tumbuh (ZPT) alami. Jurnal agroristek, 3(2): 64-72.
Fajri, K., Nopsagiarti, T., Okalia, D. 2020. Respon pertumbuhan eksplan jeruk nipis (Citrus aurantifolia L.) dengan pemberian berbagai konsentrasi ekstrak pisang raja dan arang aktif pada media ms. Jurnal Green Swarnadwipa, 9(2), 382-393.
I’anatushshoimah, nurchayati, Y., Prihastanti, E., & Hastuti, R.B 2020. Effect of Light for Callus Induction of Mangrove Plant (Rhizophora apiculata) by In Vitro. Life Sciences, 9(2).
Junairah, J., Amalia, N.S., Manuhara, S.W., Matuzzahroh, N., & Sulistyorini, L. 2019. Pengaruh Variasi Zat Pengatur Tumbuh IAA, BAP, Kinetin Terhadap Metabolit Sekunder Kalus Sirih Hitam. Jurnal Kimia Riset, 4(2): 121-132.
Latunra, A.I., Masniawati, A., Aspianti, W., & Tuwo,M. 2017. Induksi kalus pisang barangan merah Musa acuminate Colla dengan kombinasi hormone 2,4D dan Bap secara in vitro. Jurnal Ilmu Alam dan Lingkungan, 8(15), 53-61.
Luthfia, N., Rahmawati, M., & Hayati, M. 2019. Efektifitas konsentrasi NAA dan Kinetin terhadap pertumbuhan tunas pisang raja secara in vitro. Jurnal Ilmiah Mahasiswa Pertanian, 4(2), 1-8.
Mayura, E. 2020. Pengaruh Berbagai Komposisi Media Terhadap Induksi Tunas Tanaman Nilam (Pogestemon Cablin Benth). Prosiding Webinar Nasional Series Sistem Pertanian Terpadu Dalam Pemberdayaan Petani di Era New Normal, (pp. 42-58).
Piter, Y. 2021. Metode perbanyak tanaman ubi jalar ungu (Ipomea batatas Poiret) dengan teknik kultur jaringan atau stek plantlet. Jurnal Inovasi Pendidikan, 2(3), 1037-1045.
Riono, Y., 2019. Zat pengatur tumbuh kinetin untuk pertumbuhan sub kultur pisangan barangan (Musa paradisiaca. L) dengan metode kultur jaringan. Jurnal Agro Inragiri, 2(1), 23-33.
Rodinah., & Nisa, C. 2018. Formulasi zat pengatur tumbuh dengan interval waktu subkultur terhadap inisiasi dan multiplikasi pisang talas secara in vitro. Jurnal Ziraah, 4(3), 141- 148.
Samanhudi, S., Widijanto, H., & Yunus, A. 2020 Sosialisasi dan penyuluhan budidaya pisang dengan bibit hasil kultur jaringan di Desa Lempong, Kecamatan Jenawi, Kabupaten Karanganyar. PRIMA: Journal of Community Empowering and Services, 4(2), 59-63.
Sari, S.I.P., Murdiono, W.E., & Barunawati, N. 2018. Perbanyakan bibit bawang dayak (Eleutherine palmifolia L) secara in vitro. PLANTROPICAL: Journal of Agricultural Science, 3(1), 54-61.
Yeyen, Y., Nopsagiarti, T., & Seprido, D. 2021. Uji berbagai sitokinin pada media ms terhadap pertumbuhan globular eksplan pisang barangan (Musa acuminate). Jurnal Green Swarnadipa, 10(2), 176-184.
Yuslinawari, Y., Jibril, H.M., Andayani, S.T., Hidayat, S.N., & Nugraha, P.A. 2023. Pengaruh zat pengatur tumbuh sintesis IAA dan IBA pada pertumbuhan semai pronojiwo (Sterculia javanica. R. Br.). jurnal Pertanian Agros, 25(1), 59-67.
Yustisia, D., Aryad, M., Wahid, A., & Asri, J. 2019. Pengaruh pemberian ZPT alami (air kelapa) pada media ms 0 terhadap pertumbuhan planlet kentang (Solanum tuberosum. L.). jurnal Agrominasia, 3(2), 130-140.
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