Effect of exopolysaccharide-producing Azotobacter and cow manure on nutrient uptake and root-to-shoot ratio of sorghum

  • Reginawanti Hindersah Department of Soil Science, Faculty of Agriculture, Universitas Padjajaran
  • Anny Yuniarti Department of Soil Science, Faculty of Agriculture, Universitas Padjajaran
  • Hidiyah Ayu Ratna Ma’rufah Department of Soil Science, Faculty of Agriculture, Universitas Padjajaran
Keywords: Exopolysaccharide-producing Azotobacter, cow manure, nutrient uptake, Sorghum

Abstract

Nitrogen-fixing Azotobacter synthesizes exopolysaccharide, which is important among other to improve aggregate stability and hence nutrients uptake. A pot experiment has been conducted to determine the effect of exopolysaccharide-producing Azotobacter and organic matter on nitrogen, phosphor, and potassium uptake by the shoot of sorghum (Sorghum bicolor (L.) Moench), and plant growth. The pot experiment was setup in randomized block design which test eight combination treatments of Azotobacter isolates (AS5, AS6, and AS5 + AS6) and organic matter application (with and without 20 t ha-1 of cow manure). The result showed dual inoculation of Azotobacter AS5 and AS6 inoculation combined with cow manure application increased N and P uptake. The dual inoculation treatment did not affect root length; but increased the shoot height and dry weight when accompanied by the application of cow manure. The ratio of root and shoot dry weight was not influenced by single or dual Azotobacter inoculation with or without organic matter.

Downloads

Download data is not yet available.

References

Ahmad, F., Ahmad, I., & Khan, M.S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiology Research, 163(2):173-181.

Alori, E., Glick, B.R., & Babalola, O.O. (2017). Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Frontier in. Microbiology, 8(971): 1-8

AOAC. (2012). Official methods of analysis, Association of official analytical chemist 19th edition, Washington D.C., USA

Bengough, A.G., McKenzie, B.M., Hallett, P.D., & Valentine, T.A. (2011). Root elongation, water stress, and mechanical impedance: a review of limiting stresses and beneficial root tip traits. Journal of Experimental Botany 62(1):59–68.

Bodhinayake, W., Cheng Si, B., & Xiao, C. (2004). New method for determining water conducting macro- and mesoporosity from tension infiltrometer. Soil Science Society American Journal, 68:760-769.

Emtiazi, G., Ethemadifar, Z., Habibi, M.H. (2004). Production of extracellular polymer in Azotobacter and biosorption of metal by exopolymer. African Journal of Biotechnology, 3:330-333.

Galloway, A.F., Akhtar, J., Marcus, S.E., Fletcher, N., Field, K., & Knox, P. (2020). Cereal root exudates contain highly structurally complex polysaccharides with soil‐binding properties. The Plant Journal, 103(5): 1666-1678.

Guo, Y-S., Furrer, J.M., Kadilak, A.L., Hinestroza, H.F., Gage, D.J., Cho, Y.K., & Shor, L.M. (2018). Bacterial extracellular polymeric substances amplify water content variability at the pore scale. Frontier in Environmental Science, 6:93, pp 13.

Guidi, P., Falsone, F., Mare, B.T., Simoni, A., Gioacchini, P., & Vianello, G. (2013). Relating loss of soil fertility to water aggregate stability and nutrient availability in agricultural calcaric soils. Environmental quality/Qualité de l’Environnement/Qualità ambientale, 11: 01-16.

Hindersah, R., Arief, D.H., Soemitro, S., & Gunarto, L. (2006). Exopolysaccharide extraction from rhizobacteria Azotobacter sp. Proc. International Seminar IMTGT. Medan, 22-23 June 2006. pp 50-55.

Huthily, K.H., Maje, H.R., Al-deen Gaze, E.A., & AL-Qadisiyah. (2015). The effect of bio-fertilization in growth and yield four two types of sorghum (Sorghum bicolor (L.) Moench). Journal of Agriculture Sciences, 5(2):96-105. Abstract in English

Kim, S.Y., Sivaguru, M., & Stacey, G. (2006). Extracellular ATP in plants. Visualization, localization, and analysis of physiological significance in growth and signaling. Plant Physiology, 42(3):984-92.

Kizikaya R. (2009). Nitrogen fixation capacity of Azotobacter spp. strains isolated from soils in different ecosystems and relationship between them and the microbiological properties of soils. Journal of Environmental Biology, 30(1):73-82.

Mahato, S., & Kafle, A. (2018). Comparative study of Azotobacter with or without other fertilizers on growth and yield of wheat in Western hills of Nepal. Annals of Agrarian Science, 16(3), 250–256.

Minardi, S., Harieni, S., Anasrullah, A., & Purwanto, H. (2016). Soil fertility status, nutrient uptake, and maize (Zea mays L.) yield following organic matters and P fertilizer application on Andisol. IOP Conf. Series: Materials Science and Engineering, 193: 012054

Nosrati, R., Owlia, P., Saderi, H., Rasooli, I., & Ali Malboobi, M. (2014). Phosphate solubilization characteristics of efficient nitrogen fixing soil Azotobacter strains. Iranian Journal of Microbiology, 6(4), 285–295.

Oleghe, E., Naveed, M., Baggs, E.M. et al. (2017). Plant exudates improve the mechanical conditions for root penetration through compacted soils. Plant & Soil, 421:19–30

Puli, M.R., Prasad, P.R.K, Jayalakshmi, M., & Rao, B.R., (2017). Effect of organic and inorganic sources of nutrients on NPK uptake by rice crop at various growth periods. Research Journal of Agricultural Sciences, 8(1): 64-69,

Rubio, E.J., Montecchia, M.S., Tosi, M., Cassán. F.D., Perticari, A., & Correa, O.S. (2013). genotypic characterization of Azotobacteria isolated from Argentinean soils and plant-growth-promoting traits of selected strains with prospects for biofertilizer production. Science World Journal, 2013: 519603.

Sabra, W., Zeng, A.P., Lünsdorf, H., & Deckwer, W.D. (2000). Effect of oxygen on formation and structure of Azotobacter vinelandii alginate and its role in protecting nitrogenase. Applied and Environmental Microbiology, 66(9):4037-44.

Severiano, E.C., Oliveira, G.C., Dias Junior, M.S., Curi, N., Costa, K.A.P., & Carducci, C.E. (2013). Preconsolidation pressure, soil water retention characteristics, and texture of Latosols in the Brazilian Cerrado. Soil Research, 51:193-202.

Susilowati, A., Puspita, A.A., & Yunus, A. (2018). Drought resistant of bacteria producing exopolysaccharide and IAA in rhizosphere of soybean plant (Glycine max) in Wonogiri Regency Central Java Indonesia. IOP Conference Series: Earth and Environmental Science, 142:012058

Ventorino, V., Nicolaus, B., Di Donato, P., Pagliano, P., Poli, A., Robertiello, A. et al. (2019). Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse. Chemical and Biological Technology in Agriculture, 6:18.

Wijanarko, A., Purwanto, B.H., Shiddieq, Dj., & Indradewa, D. (2012). Pengaruh kualitas bahan organik dan kesuburan tanah Terhadap mineralisasi nitrogen dan serapan N oleh tanaman ubikayu di Ultisol. Jurnal Perkebunan dan Lahan Tropika, 2(2):1-14

Widodo, K.H., & Kusuma, J. (2018). Pengaruh kompos terhadap sifat fisik tanah dan pertumbuhan tanaman jagung di Inceptisol. Jurnal Tanah dan Sumberdaya Lahan, 5(2):959-967.

Yulina, H., Devnita, R., & Harryanto, R. (2018). Respon air tersedia dan bobot isi tanah pada tanaman jagung manis dan brokoli terhadap kombinasi terak baja dan bokashi sekam padi pada andisol, Lembang. Jurnal Agrikultura, 29 (2): 66-72.

Published
2021-02-26
How to Cite
Hindersah, R., Yuniarti, A., & Ma’rufah, H. A. R. (2021). Effect of exopolysaccharide-producing Azotobacter and cow manure on nutrient uptake and root-to-shoot ratio of sorghum. Jurnal Ilmiah Pertanian, 17(2), 80-85. https://doi.org/10.31849/jip.v17i2.5205
Section
Original Articles
Abstract viewed = 213 times
PDF downloaded = 200 times