Identification of root-associated arbuscular mycorrhizal fungi in cassava (Manihot esculenta Crantz) in Manokwari Regency, West Papua, Indonesia

Antonius Suparno; Darmawanta Tarigan; Yohanis Amos Mustamu; Linda Ernawati Lindongi; Wiwik Hardaningsih

doi:10.31849/jip.v20i1.11695

Antonius Suparno Department of Agrotechnology, Faculty of Agriculture, Universitas Papua, West Papua, Indonesia
Darmawanta Tarigan Department of Agrotechnology, Faculty of Agriculture, Universitas Papua, West Papua, Indonesia
Yohanis A. Mustamu Department of Agrotechnology, Faculty of Agriculture, Universitas Papua, West Papua, Indonesia
Linda E. Lindongi Department of Agrotechnology, Faculty of Agriculture, Universitas Papua, West Papua, Indonesia
Wiwik Hardaningsih Department of Food Crop Production, Payakumbuh State Polytechnic of Agriculture, West Sumatra, Indonesia

DOI: https://doi.org/10.31849/jip.v20i1.11695

Keywords: arbuscular mycorrhiza fungi, cassava, diversity, roots and rhizosphere, Manokwari

Abstract

Arbuscular mycorrhizal fungi (AMF) are crucial for plants growing on marginal soils, especially those with a very high dependence on AMF; one of them is plants with few roots, such as cassava. This study aimed to identify the AMF associated with cassava in six locations in Manokwari Regency, West Papua, Indonesia. The research was carried out using an observation method and purposive sampling at 6 locations. Soil samples were taken from the rhizosphere of 8-month-old cassava plants, with 2 kg of soil at each site. Furthermore, using the host plant Sorghum bicolor L, AMF was trapped in rhizosphere soil samples for three months. The spore morphology identification showed that cassava in North Manokwari District was associated with 3 genera 7 species, West Manokwari District-Manggoapi obtained 3 genera 7 species, Warmare District obtained 2 genera 7 species, Prafi SP-3 District obtained 3 genera 6 species, Masni SP-5 District obtained 3 genera 7 species, and Masni SP-8 District obtained 2 genera 7 species. The highest AMF colonization rate (94%) was found in the roots of the host plant Sorghum bicolor (L.) originating from Masni SP-5 District with the AMF composition consisting of Acaulospora cf. rehmi, Acaulospora cf. spinosa, Acaulospora cf. gerdemanii, Glomus cf. clarum, Gigaspora cf. margarita, Gigaspora cf. rosea, and Gigaspora cf. gigantea.

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References

Ahanger, M. A., Tyagi, S. R., Wani, M. R., & Ahmad, P. (2014). Drought tolerance: role of organic osmolytes, growth regulators, and mineral nutrients. In P. Ahmad, M. R. Wani (Eds.), Physiological mechanisms and adaptation strategies in plants under changing environment (vol. 1., pp. 25–55). Springer. https://doi.org/10.1007/978-1-4614-8591-9_2

Anggraeny, Y., Nazip, K., & Santri, D. J. (2017). Identifikasi Fungi Mikoriza Arbuskula (FMA) pada Rhizosfer Tanaman di Kawasan Revegetasi Lahan Penambangan Timah di Kecamatan Merawang Kabupaten Bangka dan Sumbangannya pada Pembelajaran Biologi SMA. Prosiding Seminar Nasional Pendidikan SAINS IPA, 1(1), 391-403.

Auli, N.R. & Kasiamdari, R.S.. (2019). Produksi Inokulum Vesikular Arbuskular Mikoriza Pada Inang Sorghum bicolor (L.) Moench dengan Variasi Jenis Inokulum dan Pupuk NPK. J. Riset Biologi dan Aplikasinya, 1(2), 80-86. https://doi.org/10.26740/jrba.v1n2.p80-86

Begoude, D.A.B., P.S. Sarr, T.L.Y. Mpon, D.A. Owona, M.N. Kapeua & S. Araki. (2016). Composition of Arbuscular Mycorrhizal Fungi Associated with Cassava (Manihot esculenta Crantz) Cultivar as Influenced by Chemical Fertilization and Tillage in Cameroon. Journal of Applied Biosciences, 98, 9270-9283.

Brundrett, M.C. (2009). Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant and Soil, 320, 37–77.

Ceballos, I., Mateus, I. D., Peña, R., Peña-Quemba, D. C., Robbins, C., Ordoñez, Y. M., Rosikiewicz, P., Rojas, E.C., Thuita, M., Mlay, D. P., Masso, C., Vanlauwe, B., Rodriguez, A. & Sanders, I. R. (2019). Using variation in arbuscular mycorrhizal fungi to drive the productivity of the food security crop cassava. bioRxiv [Preprint], https://doi.org/10.1101/830547

Ceballos, I., Michael, R., Christhian, R., Ricardo, F., Alia, P. R., & Ian R.S. (2013). The In Vitro Mass-Produced Model Mycorrhizal Fungus, Rhizophagus Irregularis, Significantly Increase Yields of the Globally Important Food Security Crop Cassava. Plos One, 8(8), 1-10.

Deguchi, S., Yosuke, M., Chisato, T., Yuki, S., Hajime, O., & Yoshimune, O. (2017). Proposal of a New Estimation Method of Colonization Rate of Arbuscular Mycorrhizal Fungi in the Roots of Chengiopanax sciadophylloides. Mycobiology, 45(1), 15-19. https://doi.org/10.5941/MYCO.2017.45.1.15

Fall, A. F., Grace, N., Joseph, S., Hassna, F., Samuel, O. A., Abibatou, N., Arfang, B., & Khady, N. (2022). Roles of Arbuscular Mycorrhizal Fungi on Soil Fertility: Contribution in the Improvement of Physical, Chemical, and Biological Properties of the Soil. Front. Fungal Biol., (3), 723892. doi: 10.3389/ffunb.2022.723892

Fitriani, B. M., Hindersah, R., & Suryatmana, P. (2008). Aktivitas Enzim Fosfatase dan Kertersediaan Fosfat Tanah Pada Sistem Tumpang Sari Tanaman Pangan dengan Jati (Tectona grandis L.f.) Setelah Aplikasi Pupuk Hayati. Jurnal Agrikultura, 19(3),161-166

Gianinazzi, S., Gollotte, A., Binet, M.N., van Tuinen, D., Redecker, D. & Wipf, D. (2010). Agroecology: the key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza, (20), 519–530.

Hanudin, E., Iskyati, W., & Yuwono, N.W. (2021). Improving national value of cow manure with biomass ash and its response to the growth and K-Ca absorption of Mustard on Inceptisols. IOP Conference Series Earth and Environmental Science, (75), 1-9. https://doi.org/10.1088/1755-1315/752/1/012015

Husein, M., Umami, N., & Pertiwiningrum, A. (2021). Pengaruh Inokulasi Fungi Mikoriza Arbuskula Indigenous Bambusa Sp, Cichorium Intybus L, Pinus merkusii Terhadap Pertumbuhan, Produktivitas dan Kandungan Nutrien Hijauan Cichorium Intybus L. [Master thesis, Universitas Gadjah Mada].

Kiheri, H., Jussi, H., & Sari, T. (2016). Staining and microscopy of mycorrhizal fungal colonization in preserved ericoid plant roots. Journal of Berry Research, 7, 231–237. https://doi.org/10.3233/JBR-170160

Koske, R.E. & Gemma, J.N. (1989). A Modified Procedure for Staining Roots to Detect VA Mycorrhizas. Mycological Research, 92, 486-488. http://dx.doi.org/10.1016/S0953-7562(89)80195-9.

Lestari, T., Apriyadi, R., & Setiawan, F. (2018). Keragaan tanaman ubi kayu lokal Bangka dengan pemberian mikoriza di tanah asam. Agrosainstek, 2(1), 15-21.

Miyasaka, S.C. & Habte, M. (2007). Plant Mechanisms and Mycorrhizal Symbioses to Increase Phosphorus Uptake Efficiency. Communications in Soil Science and Plant Analysis, 32, 7-8, 1101-1147, https://doi.org/10.1081/CSS-100104105

Nusantara, A.D., R.Y.H. Bertham & I. Mansur. (2012). Bekerja dengan Fungi Mikoriza Arbuskula. IPB Press. Bogor.

Prasad, K. (2020). Positive Importance of Arbuscular Mycorrhizal Fungi for Global Sustainable Agriculture and Environment Management for Green Technology. Short Communication. Current Investigations in Agriculture and Current Research, 9(2). https://doi.org/10.32474/CIACR.2020.09.000306

Priyono (2016). Metode Penelitian Kuantitatif. Zifatama Publishing. Surabaya.

Redecker, D., Schüssler, A., Stockinger, H., Stürmer, S. L., Morton, J. B., & Walker, C. (2013). An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota). Mycorrhiza 23(7), 515–531. https://doi.org/10.1007/s00572-013-0486-y

Sarr, P.S., Sugiyama, A., Begoude, , A.D.B., Yazaki, K., Shigeru, A. S., & EijiNawata, E. (2019). Diversity and distribution of Arbuscular Mycorrhizal Fungi in cassava (Manihot esculenta Crantz) croplands in Cameroon as revealed by Illumina MiSeq. Rhizosphere;Volume 10. 100147. https://doi.org/10.1016/j.rhisph.2019.100147

Selosse, M. A., Strullu-Derrien, C., Martin, F. M., Kamoun, S., & Kenrick, P. (2015). Plants, fungi, and oomycetes: a 400-million years affair that shapes the biosphere. New Phytol., 206, 501–506. https://doi.org/10.1111/nph.13371

Smith, S.E., & Read, D. J. (2008). Mycorrhizal Symbiosis. 3rd ed. San Diego: Academic Press.

Soedarjo. M. (2019). Mutualistic Symbiosis between Arbuscular Visicular Mycorrhizal Fungi with Cassava Plant (Manihot esculenta Crantz). International Journal of Research Studies in Agricultural Sciences (IJRSAS), 5(11), 8-17. http://dx.doi.org/10.20431/2454-6224.0511002.

Suharno, V., Agustini, Tanjung, R. H. R., & Sufaati, S. (2019). Fungi Mikoriza Arbuskula (FMA) yang Berasosiasi dengan Durio zibethinus di Kabupaten Manokwari, Papua Barat, Indonesia. Jurnal Pemuliaan Tanaman Hutan, 13(2), 61 – 69.

Supriyanto. (2019). Solusi Tanah Pertanian Keracunan Aluminium dan Besi. cyber extension - Pusluhtan Kementan. https://indonesiabertanam.com/2015/01/03/solusi-lahan-pertanian-keracunan-al-dan-fe/

Taberima, S., Fenetiruma, O.A., Muzendi, A.S.M., Bachri, S., Baransano, M. A., Syamsudin, K., Ranti, F. D., Sala, R., Matulessy, M., Lindongi, L. E., Mawikere, N. L., & Palulungan, J. A. (2020). Kesesuaian Lahan dan Komoditas Unggulan Kabupaten Manokwari. Fakultas Pertanian Universitas Papua. e-ISBN: 978-623-95419-1-0

Vizzini, A., Giovanni, C., & Ledo, S. (2020). Testing spore amyloidity in Agaricales under light microscope: the case study of Tricholom. IMMA Fungus, 11:24. https://doi.org/10.1186/s43008-020-00046-8

Widiatma, Sena, P., Wirawan, I. G. P., & Susrama, I. G. K. (2015). Identifikasi Mikoriza Vesikular Arbuskular (MVA) pada Rhizosfer Tanaman Ubi Jalar (Ipomoea batatas L.) dan Ubi Kayu (Manihot esculenta Crantz) serta Perbanyakannya dengan Media Zeolit. Jurnal Agroekoteknologi Tropika 4 (4), 2301-6515.