Preliminary in vitro inhibitory activity of a combined acetogenin–curcumin extract against Fusarium spp.

Nurhamdana Nurhamdana; Iswanto Iswanto; Nur Dzakira  Sahbani; Muahra Dwi  Putri; Rifky   Ramadhan; Muh. Kurnia Qadri  Ramadhan Z.; Ali Hasan  Salman

doi:10.31849/jip.v22i3.29777

Authors

Nurhamdana Nurhamdana Department of Forestry, Faculty of Forestry, Hasanuddin University, Makassar 90245, Indonesia
Iswanto Iswanto Department of Forestry, Faculty of Forestry, Hasanuddin University, Makassar 90245, Indonesia
Nur Dzakira Sahbani Department of Forestry, Faculty of Forestry, Hasanuddin University, Makassar 90245, Indonesia
Muahra Dwi Putri Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar 90245, Indonesia
Rifky Ramadhan Department of Forestry, Faculty of Forestry, Hasanuddin University, Makassar 90245, Indonesia
Muh. Kurnia Qadri Ramadhan Z. Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar 90245, Indonesia
Ali Hasan Salman Department of Forestry, Faculty of Forestry, Hasanuddin University, Makassar 90245, Indonesia

DOI:

https://doi.org/10.31849/jip.v22i3.29777

Keywords:

botanical antifungal agents, plant-derived bioactive compounds, soil-borne fungal pathogens, in vitro inhibition assay, chilli crop protection

Abstract

Fusarium spp. are among the most destructive soil-borne fungal pathogens affecting chilli production and remain difficult to control using conventional approaches. This study reports a preliminary in vitro observation of the inhibitory activity of a combined acetogenin–curcumin extract against Fusarium spp. Acetogenin was obtained from soursop leaves (Annona muricata L.), while curcumin was derived from turmeric (Curcuma longa L.). The study involved extract preparation, fungal isolation, functional group identification, and antifungal assays using different extract ratios. Morphological observations confirmed that the isolated pathogen was consistent with Fusarium spp. Among the tested formulations, the extract combination containing 60% acetogenin and 40% curcumin showed the highest inhibitory activity, reducing fungal colony growth by up to 84% compared to the control. These findings provide an initial indication that a combined acetogenin–curcumin extract may enhance in vitro inhibition of Fusarium spp. and support further investigation using replicated assays and extended evaluations.

References

Abadi, A. L., Syib’li, M. A., Aini, L. Q., Sektiono, A. W., Choliq, F. A., & Trianti, I. (2023). Pengelolaan Penyakit Tumbuhan Terpadu (Integrated Plant Disease Management). Universitas Brawijaya Press. https://doi.org/10.21070/2020/978-623-6833-08-7

Balouiri, M., Sadiki, M., & Saad, I. (2015). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6. https://doi.org/10.1016/j.jpha.2015.11.005

Cahyani, A., Anggraini, D. I., Soleha, T. U., & Tjiptaningrum, A. (2020). Uji Efektivitas Antibakteri Ekstrak Rimpang Kunyit (Curcuma domestica Val.) terhadap Pertumbuhan Propionibacterium acnes In Vitro. Jurnal Kesehatan, 11(3), 414-421. https://doi.org/10.26630/jk.v11i3.2241

Central Statistics Agency/Badan Pusat Statistik Indonesia. (2023, June 9). Statistik Hortikultura 2022. https://www.bps.go.id/id/publication/2023/06/09/03847c5743d8b6cd3f08ab76/statistik-hortikultura-2022.html.

Chen, C., Long, L., Zhang, F., Chen, Q., Chen, C., Yu, X., Liu, Q., Bao, J., & Long, Z. (2018). Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum. PLOS ONE, 13(3), e0194284. https://doi.org/10.1371/journal.pone.0194284

Ciuca, M. D., & Racovita, R. C. (2023). Curcumin: Overview of Extraction Methods, Health Benefits, and Encapsulation and Delivery Using Microemulsions and Nanoemulsions. International Journal of Molecular Sciences, 24(10). https://doi.org/10.3390/ijms24108874

David, W., Alkausar, S., & Widyarti, B. (2023). Statistik Pertanian Organik Indonesia. Universitas Bakrie Press. https://repository.bakrie.ac.id/id/eprint/8918

Dewi, N. K. A. C. P. (2024). Potensi Senyawa Kurkumin Tanaman Kunyit (Curcuma longa L.) sebagai Antioksidan untuk Menurunkan Perkembangan Sel Kanker. Prosiding Workshop Dan Seminar Nasional Farmasi, 3, 197–205. https://doi.org/10.24843/WSNF.2024.v03.p19

Dong, H., Wang, Y., Peng, X., Zhou, H., Zhao, F., Jiang, Y., Zhang, D., & Jin, Y. (2021). Synergistic antifungal effects of curcumin derivatives as fungal biofilm inhibitors with fluconazole. Chemical Biology & Drug Design, 97(5), 1079–1088. https://doi.org/10.1111/cbdd.13827

Harish, J., Jambhulkar, P. P., Bajpai, R., Arya, M., Babele, P. K., Chaturvedi, S. K., Kumar, A., & Lakshman, D. K. (2023). Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize. Frontiers in Microbiology, 14, 1121781. https://doi.org/10.3389/fmicb.2023.1121781

Hörmanseder, K., Obermeyer, G., & Foissner, I. (2005). Disturbance of endomembrane trafficking by brefeldin A and calyculin A reorganizes the actin cytoskeleton of Lilium longiflorum pollen tubes. Protoplasma, 227(1), 25–36. https://doi.org/10.1007/s00709-005-0132-4

Indrayati, S., & Rosalina, S. (2020). Uji Efektivitas Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Pertumbuhan Jamur Candida albicans. Prosiding Seminar Kesehatan Perintis, 3(2), 1–7. https://jurnal.upertis.ac.id/index.php/PSKP/article/view/627

Jamin, F. S., Mustofa, D., Restu, K., Rusli, M., & Adhi, S. (2024). Penggunaan Pestisida dalam Pertanian : Resiko Kesehatan dan Alternatif Ramah Lingkungan Pesticide Use in Agriculture : Health Risks and Environmentally Friendly Alternatives. 7(11), 4151–4159. https://doi.org/10.56338/jks.v7i11.6342

Jeong, J., Jung, I., Yum, S., Hwang, Y., Jeong, J., Jung, I., Yum, S., & Hwang, Y. (2023). In Vitro Synergistic Inhibitory Effects of Plant Extract Combinations on Bacterial Growth of Methicillin-Resistant Staphylococcus aureus Pharmaceuticals, 16(30). https://doi.org/10.3390/ph16101491

Le Dang, Q., Nguyen, C. Q., Vo, T. K. A., Nguyen, T. T. T., Pham, Q. D., Nguyen, T. X., Cao, T. H., De Tran, Q., Le, T. T., & Do, T. H. (2024). A botanical nanoemulsion against phytopathogenic fungi Colletotrichum sp. and Fusarium oxysporum: Preparation, in vitro and in vivo bioassay. Journal of Natural Pesticide Research, 10, 100099. https://doi.org/10.1016/j.napere.2024.100099

Leslie, J. F., & Summerell, B. A. (2006). Fusarium laboratory workshops—A recent history. Mycotoxin Research, 22(2), 73–74. https://doi.org/10.1007/BF02956766

Lestari, A., Henri, H., Sari, E., & Wahyuni, T. (2021). Microscopic Characterization of Fusarium sp. associated with yellow disease of pepper (Piper nigrum L.) in South Bangka Regency. Planta Tropika, 9(1), 1-9. https://doi.org/10.18196/pt.v9i1.7753

Lim, T., Rialita, A., & Mahyarudin, M. (2022). Aktivitas Antijamur Isolat Bakteri Endofit Tanaman Kunyit Terhadap Penghambatan Pertumbuhan Malassezia furfur secara in-vitro. Jurnal Ilmiah Umum dan Kesehatan Aisyiyah, 7(1), 1–11. https://doi.org/10.35721/jakiyah.v7i1.138

Mayadianti, I.A.I., Khalimi, K., & Suniti, N.W. (2020). Uji daya hambat bakteri Paenibacillus polymyxa terhadap pertumbuhan jamur Colletotrichum sp. secara in vitro. Jurnal Agroekoteknologi Tropika, 9(4), 229-237. https://ojs.unud.ac.id/index.php/jat/article/view/68521

Mukharomah, S. (2019). Potensi senyawa asetogenin dari daun sirsak (Annona Muricata Linn.) yang diembankan pada zeolit NaX sebagai senyawa antikanker payudara T47D [Unpublished thesis dissertation]. Universitas Islam Negeri Maulana Malik Ibrahim. http://etheses.uin-malang.ac.id/id/eprint/15215

Nasikh, N. Utomo, S.H., Wardana, L.W., Astawa, I. P. (2023). Pengembangan Potensi Lahan Pertanian Berbasis Green Economy Untuk Ketahanan Pangan Berkelanjutan. Uwais Inspirasi Indonesia.

Neglo, D., Tettey, C. O., Essuman, E. K., Amenu, J. D., Mills-Robertson, F. C., Sedohia, D., Boakye, A. A., & Abaye, D. A. (2021). Evaluation of the Modulatory Effect of Annona muricata Extracts on the Activity of Some Selected Antibiotics against Biofilm-Forming MRSA. Evidence-Based Complementary and Alternative Medicine : ECAM, 2021, 9342110. https://doi.org/10.1155/2021/9342110

Pasaribu, T. (2019). Peluang zat bioaktif tanaman sebagai alternatif imbuhan pakan antibiotik pada ayam. Jurnal Litbang Pertanian, 38(2), 96-104. https://doi.org/10.21082/jp3.v38n2.2019.p96-104

Poyer, S., Laboureur, L., Hebra, T., Elie, N., Van der Rest, G., Salpin, J.-Y., Champy, P., & Touboul, D. (2022). Dereplication of Acetogenins from Annona muricata by Combining Tandem Mass Spectrometry after Lithium and Copper Postcolumn Cationization and Molecular Networks. Journal of the American Society for Mass Spectrometry, 33(4), 627–634. https://doi.org/10.1021/jasms.1c00303

Septian, R. D., Afifah, L., Surjana, T., Saputro, N. W., & Enri, U. (2021). Identifikasi dan efektivitas berbagai teknik pengendalian hama baru ulat grayak Spodoptera frugiperda JE Smith pada tanaman jagung berbasis PHT-biointensif. Jurnal Ilmu Pertanian Indonesia, 26(4), 521–529. https://doi.org/10.18343/jipi.26.4.521

Singh, P. K., Singh, J., Medhi, T., & Kumar, A. (2022). Phytochemical Screening, Quantification, FT-IR Analysis, and In Silico Characterization of Potential Bio-active Compounds Identified in HR-LC/MS Analysis of the Polyherbal Formulation from Northeast India. ACS Omega, 7(37), 33067–33078. https://doi.org/10.1021/acsomega.2c03117

Ulhaq, M. A., & Masnilah, R. (2019). Pengaruh penggunaan beberapa varietas dan aplikasi Pseudomonas fluorescens untuk mengendalikan penyakit bulai (Peronosclerospora maydis) pada tanaman jagung (Zea mays L.). Jurnal Pengendalian Hayati, 2(1), 1–9. https://doi.org/10.19184/jph.v2i1.17131

Wilisiani, F., Sumardi, S., & Hasibuan, A. (2020). Potensi Fusarium sp. Dari Tanaman Pisang Sebagai Inokulan Pembentukan Gaharu. Jurnal Pertanian Agros, 22(2), 253–257. https://doi.org/http://dx.doi.org/10.35941/jatl.6.2.2024.14059.18-22

Zahro, S. F., Lailatul Restuning Putri Istiqomah, Galuh Citra Cahya Rohmana, Denise Ayu Yustikaningrum, & Christyfani Sindhuwati. (2023). Potensi Daun Sirsak (Annona Muricata L.) sebagai Kandidat Fungisida Nabati Penghambat Pertumbuhan Cendawan Patogen Colletotrichum Gloeosporioides. Jurnal Riset Kimia, 14(1 SE-Articles), 94–106. https://doi.org/10.25077/jrk.v14i1.590